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Age-related changes in human blood lymphocyte subpopulations / Frank Hulstaert in Clinical immunology and immunopathology, 70(1994)02 ([02/01/1994])
[article] Age-related changes in human blood lymphocyte subpopulations : II. Varying Kinetics of Percentage and Absolute Count Measurements [printed text] / Frank Hulstaert, Author ; I. Hannet, Author ; V. Deneys, Author ; V. Munhyeshuli, Author ; T. Reichert, Author ; M. De Bruyere, Author ; K. Strauss, Author . - 1994 . - 152-158.
Languages : English (eng)
in Clinical immunology and immunopathology > 70(1994)02 [02/01/1994] . - 152-158
W 1 Serials. Periodicals
Adolescent ; Adult ; Aging ; Antigens, CD ; Antigens, Differentiation ; Child ; Child, Preschool ; Female ; Humans ; Immunophenotyping ; Infant ; Infant, Newborn ; Journal Article ; Leukocyte Count ; Male ; Middle Aged ; T-Lymphocytes ; United States
Abstract: A reference range for lymphocyte populations, with particular emphasis on T lymphocyte subsets, was obtained for normal individuals covering age cohorts from birth through adulthood. This report confirms and extends findings from a developmental reference range published earlier (1). Absolute numbers of WBC, lymphocytes, and T, B, and NK subsets decline significantly during childhood. However, differences in the rate of decline of certain lymphocyte subsets leads to discordance between absolute numbers and percentages. Those lymphocyte subsets which decline less rapidly with age than the total lymphocyte count will show an increase in percentage, whereas those which decline more rapidly will show further declines in percentage values. T cell percentages were seen to increase over time whereas B cell percentages decline. Markers of immaturity such as CD45RA on CD4 cells and CD38 on CD8 cells declined in both percentages and absolute numbers. Activation markers, such as HLA-DR on CD8 cells and IL2-R on CD3 cells, increased in percentages with time but changed inconsistently in cell number from infancy to adulthood. These findings extend the lymphocyte references range to markers thought to be informative in various disease states, including HIV infection. Link for e-copy: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCK-45R79S2-9&_user=1 [...] Format of e-copy: VDIC IP recognition Record link: [article]Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection / L. Kestens in AIDS, 6(1992)8 ([08/01/1992])
[article] Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection [printed text] / L. Kestens, Author ; G. Vanham, Author ; P. Gigase, Author ; G. Young, Author ; I. Hannet, Author ; F. Vanlangendonck, Author ; Frank Hulstaert, Author ; B.A. Bach, Author . - 1992 . - 793-7.
Languages : English (eng)
in AIDS > 6(1992)8 [08/01/1992] . - 793-7
W 1 Serials. Periodicals
Antigens, CD ; Antigens, Differentiation ; Fluorescent Antibody Technique ; HIV Infections ; Humans ; Journal Article ; Membrane Glycoproteins ; Peer Review ; T-Lymphocytes ; United States
Abstract: OBJECTIVE: To study the expression of the activation markers human leukocyte antigen (HLA)-DR and CD38 antigen on CD8+ T-lymphocytes in HIV-infected subjects and HIV-negative controls.
DESIGN: Two- and three-colour flow-cytometric analysis.
METHODS: Fresh peripheral venous blood was obtained from 16 HIV-infected subjects, representing four different stages of HIV disease, and from six HIV-negative controls. Three-colour lymphocyte immunophenotyping was performed using peridinyl chlorophyll-A protein (PerCP)-conjugated anti-CD8 monoclonal antibody (MAb) in combination with anti-HLA-DR (phycoerythrin) and anti-CD38 (fluorescein isothiocyanate) MAb.
RESULTS: The relative percentage of the lymphocyte populations thus defined differed between HIV-negative and HIV-positive subjects and between HIV-infected subjects at different clinical stages of disease. Simultaneous expression of HLA-DR and CD38 within the CD8 T-lymphocyte compartment increased from 8% in controls to 49% in asymptomatic HIV-infected subjects (P less than 0.005). Symptomatic patients differed from asymptomatic seropositives by a further increase in the HLA-DR+ CD38+ CD8 subset. In AIDS patients, the HLA-DR+ CD38- CD8 subset decreased (P less than 0.05) and the HLA-DR- CD38+ CD8 subset increased (P less than 0.05), compared with the other HIV disease stage patients.
CONCLUSION: There is a stage-associated pattern of HLA-DR and CD38 expression on CD8 T-lymphocytes during HIV infection; specific phenotypic patterns may have functional correlates in the host response to the virus.
Link for e-copy: http://journals.lww.com/aidsonline/Abstract/1992/08000/Expression_of_activation_ [...] Format of e-copy: IP recognition ((via CEBAM - personal login recquired) Record link: [article]