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Age-related changes in human blood lymphocyte subpopulations / F. Erkeller-Yuksel in The Journal of Pediatrics, 120(1996)2 ([02/01/1996])  

[article] Age-related changes in human blood lymphocyte subpopulations [printed text] / F. Erkeller-Yuksel, Author ; V. Deneys, Author ; B. Yuksel, Author ; I. Hannet, Author ; Frank Hulstaert, Author ; C. Hamilton, Author . - 1996 . - 216-222. Languages : English (eng) in The Journal of Pediatrics > 120(1996)2 [02/01/1996] . - 216-222 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Aged ; Aging ; Antigens, CD ; Child ; Child, Preschool ; Female ; Fetal Blood ; Humans ; Immunophenotyping ; Infant ; Journal Article ; Leukocyte Count ; Lymphocyte Subsets ; Male ; Middle Aged ; Peer Review ; United States Abstract: Flow cytometric analysis of major lymphocyte populations and their subsets reveals age-related changes in the cellular human immune system. Immunophenotypic markers were evaluated in 110 normal pediatric subjects, divided into groups of newborn infants, infants aged 2 days to 11 months, and children aged 1 to 6 years and 7 to 17 years; results were then compared with those obtained from 101 normal adults aged 18 to 70 years. Comparisons among age groups from newborn infants through adults reveal progressive declines in the absolute numbers of leukocytes, total lymphocytes, and T, B, and natural killer (NK) cells. The percentages of T cells within the total lymphocyte population increase with age, in both CD4+ and CD8+ subsets. Percentages of B and NK cells are higher in newborn infants than in adults. The expression of the activation markers interleukin-2R and HLA-DR on T cells increases with age, as does the NK-associated expression of CD57 on CD8 cells. The proportions of B lymphocytes that coexpress CD5 or CDw78 decrease with age, whereas expression of Leu-8 and CD23 increases. The proportion of CD4 cells bearing the CD45RA and Leu-8 markers is consistently lower in adults than in children. These data may serve as a reference range for studies of pediatric subjects. Link for e-copy: http://www.sciencedirect.com/science/journal/00223476 Format of e-copy: PDF [Requires Subscription] Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2187 [article]

Age-related changes in human blood lymphocyte subpopulations / Frank Hulstaert in Clinical immunology and immunopathology, 70(1994)02 ([02/01/1994])  

[article] Age-related changes in human blood lymphocyte subpopulations : II. Varying Kinetics of Percentage and Absolute Count Measurements [printed text] / Frank Hulstaert, Author ; I. Hannet, Author ; V. Deneys, Author ; V. Munhyeshuli, Author ; T. Reichert, Author ; M. De Bruyere, Author ; K. Strauss, Author . - 1994 . - 152-158. Languages : English (eng) in Clinical immunology and immunopathology > 70(1994)02 [02/01/1994] . - 152-158 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Aging ; Antigens, CD ; Antigens, Differentiation ; Child ; Child, Preschool ; Female ; Humans ; Immunophenotyping ; Infant ; Infant, Newborn ; Journal Article ; Leukocyte Count ; Male ; Middle Aged ; T-Lymphocytes ; United States Abstract: A reference range for lymphocyte populations, with particular emphasis on T lymphocyte subsets, was obtained for normal individuals covering age cohorts from birth through adulthood. This report confirms and extends findings from a developmental reference range published earlier (1). Absolute numbers of WBC, lymphocytes, and T, B, and NK subsets decline significantly during childhood. However, differences in the rate of decline of certain lymphocyte subsets leads to discordance between absolute numbers and percentages. Those lymphocyte subsets which decline less rapidly with age than the total lymphocyte count will show an increase in percentage, whereas those which decline more rapidly will show further declines in percentage values. T cell percentages were seen to increase over time whereas B cell percentages decline. Markers of immaturity such as CD45RA on CD4 cells and CD38 on CD8 cells declined in both percentages and absolute numbers. Activation markers, such as HLA-DR on CD8 cells and IL2-R on CD3 cells, increased in percentages with time but changed inconsistently in cell number from infancy to adulthood. These findings extend the lymphocyte references range to markers thought to be informative in various disease states, including HIV infection. Link for e-copy: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCK-45R79S2-9&_user=1 [...] Format of e-copy: VDIC IP recognition Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2209 [article]

The significance of activation markers on CD8 lymphocytes in human immunodeficiency syndrome: staging and prognostic value / M. Levacher in Clinical and experimental immunology, 90(1992)3 ([12/01/1992])  

[article] The significance of activation markers on CD8 lymphocytes in human immunodeficiency syndrome: staging and prognostic value [printed text] / M. Levacher, Author ; Frank Hulstaert, Author ; S. Tallet, Author ; S. Ullery, Author ; J.J. Pocidalo, Author ; B.A. Bach, Author . - 1992 . - 376-382. Languages : English (eng) in Clinical and experimental immunology > 90(1992)3 [12/01/1992] . - 376-382 Descriptors: Classification W 1 Serials. Periodicals Indexation Acquired Immunodeficiency Syndrome ; Adult ; Aged ; AIDS-Related Complex ; Antigens, CD ; Biological Markers ; Female ; Great Britain ; HIV Infections ; HIV Seropositivity ; Humans ; Immunologic Deficiency Syndromes ; Immunophenotyping ; Journal Article ; Lymphocytes ; Male ; Middle Aged ; Peer Review ; Prognosis Abstract: The objective of this prospective cohort study was to evaluate the expression of activation markers on CD8 lymphocytes at various clinical stages of HIV infection and to determine the value of these markers in identifying patients likely to have rapidly progressive disease. One hundred and three HIV+ patients, divided into four disease stages, and 34 seronegative controls were evaluated at study entry using flow cytometric immunophenotyping. The HIV patients were followed clinically for disease progression during the following 2 years. CD8 cell numbers and percentage of lymphocytes are increased after HIV infection. Expression of the CD38, HLA-DR and CD57 markers on CD8 cells was significantly increased in asymptomatic HIV-infected patients when compared with controls, as was the CD8 cell population which did not coexpress Leu-8. These activation markers were observed to be further increased in patient groups with more clinically advanced infection. The percentage of CD38 on CD8 cells emerged not only as a discriminator of disease severity, but was a strong predictor of progression in asymptomatic, lymphadenopathy and ARC patients. Given the utility of activation markers on CD8 lymphocytes in staging disease and predicting clinical outcome, the measurement of these parameters should be considered in the monitoring and management of HIV patients. Link for e-copy: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1554578/pdf/clinexpimmunol00044-0024 [...] Format of e-copy: VDIC IP recognition (1966 to embargo 1 year) Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2207 [article]