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Age-related changes in human blood lymphocyte subpopulations / F. Erkeller-Yuksel in The Journal of Pediatrics, 120(1996)2 ([02/01/1996])  

[article] Age-related changes in human blood lymphocyte subpopulations [printed text] / F. Erkeller-Yuksel, Author ; V. Deneys, Author ; B. Yuksel, Author ; I. Hannet, Author ; Frank Hulstaert, Author ; C. Hamilton, Author . - 1996 . - 216-222. Languages : English (eng) in The Journal of Pediatrics > 120(1996)2 [02/01/1996] . - 216-222 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Aged ; Aging ; Antigens, CD ; Child ; Child, Preschool ; Female ; Fetal Blood ; Humans ; Immunophenotyping ; Infant ; Journal Article ; Leukocyte Count ; Lymphocyte Subsets ; Male ; Middle Aged ; Peer Review ; United States Abstract: Flow cytometric analysis of major lymphocyte populations and their subsets reveals age-related changes in the cellular human immune system. Immunophenotypic markers were evaluated in 110 normal pediatric subjects, divided into groups of newborn infants, infants aged 2 days to 11 months, and children aged 1 to 6 years and 7 to 17 years; results were then compared with those obtained from 101 normal adults aged 18 to 70 years. Comparisons among age groups from newborn infants through adults reveal progressive declines in the absolute numbers of leukocytes, total lymphocytes, and T, B, and natural killer (NK) cells. The percentages of T cells within the total lymphocyte population increase with age, in both CD4+ and CD8+ subsets. Percentages of B and NK cells are higher in newborn infants than in adults. The expression of the activation markers interleukin-2R and HLA-DR on T cells increases with age, as does the NK-associated expression of CD57 on CD8 cells. The proportions of B lymphocytes that coexpress CD5 or CDw78 decrease with age, whereas expression of Leu-8 and CD23 increases. The proportion of CD4 cells bearing the CD45RA and Leu-8 markers is consistently lower in adults than in children. These data may serve as a reference range for studies of pediatric subjects. Link for e-copy: http://www.sciencedirect.com/science/journal/00223476 Format of e-copy: PDF [Requires Subscription] Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2187 [article]

Two-color Immuno-fluorescence and Flow Cytometric Analysis of Peripheral Blood Lymphocyte Subsets in Caucasian and Japanese Healthy Subjects / M. Senju in Japanese journal of medicine, 30(1991)06 ([10/01/1991])  

[article] Two-color Immuno-fluorescence and Flow Cytometric Analysis of Peripheral Blood Lymphocyte Subsets in Caucasian and Japanese Healthy Subjects [printed text] / M. Senju, Author ; K. Makiyama, Author ; K. Hara, Author ; Frank Hulstaert, Author ; N. Lowder, Author ; D.P. Jewell, Author . - 1991 . - 509-15. Languages : English (eng) in Japanese journal of medicine > 30(1991)06 [10/01/1991] . - 509-15 Descriptors: Classification W 1 Serials. Periodicals Indexation Adult ; Antigens ; Asian Continental Ancestry Group ; European Continental Ancestry Group ; Female ; Flow Cytometry ; Fluorescent Antibody Technique ; Humans ; Japan ; Journal Article ; Lymphocyte Subsets ; Male ; Middle Aged Abstract: Two-color immunofluorescence using multiparameter flow cytometry was employed to examine the antigenic characteristics of peripheral blood lymphocytes in whole blood of healthy Caucasians and Japanese. The CD4/CD8 ratio in Japanese was significantly decreased compared with that in Caucasians, because of the increased number of CD8+ cells. Although the proportions of suppressor-inducer T cells (CD4+, Leu-8+) and helper-inducer T cells (naive T cells) (CD4+, CD45RA-) were low in Japanese subjects, there were no differences in the absolute numbers of suppressor-inducer T cells and helper-inducer T cells (naive T cells) in circulation. The level of activated T cells in Japanese was similar to that in Caucasians. NK cells, CD57+, CD8+ cells and CD57+, CD3+ cells were high in Japanese. Regarding B cell subsets, CD5+ B cells and activated B cells remained unchanged. However, there were slight differences in Leu-8+ B cells and Fc epsilon R+ B cells (CD20+, CD23+) between the two groups. Thus, a differing influence of racial and environmental background between healthy Caucasians and healthy Japanese on human lymphocyte subsets is present in the lymphocyte immunophenotype. Link for e-copy: http://www.journalarchive.jst.go.jp/jnlpdf.php?cdjournal=internalmedicine1962&cd [...] Format of e-copy: PDF [Open Access] Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2185 [article]