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Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection / L. Kestens in AIDS, 6(1992)8 ([08/01/1992])  

[article] Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection [printed text] / L. Kestens, Author ; G. Vanham, Author ; P. Gigase, Author ; G. Young, Author ; I. Hannet, Author ; F. Vanlangendonck, Author ; Frank Hulstaert, Author ; B.A. Bach, Author . - 1992 . - 793-7. Languages : English (eng) in AIDS > 6(1992)8 [08/01/1992] . - 793-7 Descriptors: Classification W 1 Serials. Periodicals Indexation Antigens, CD ; Antigens, Differentiation ; Fluorescent Antibody Technique ; HIV Infections ; Humans ; Journal Article ; Membrane Glycoproteins ; Peer Review ; T-Lymphocytes ; United States Abstract: OBJECTIVE: To study the expression of the activation markers human leukocyte antigen (HLA)-DR and CD38 antigen on CD8+ T-lymphocytes in HIV-infected subjects and HIV-negative controls. DESIGN: Two- and three-colour flow-cytometric analysis. METHODS: Fresh peripheral venous blood was obtained from 16 HIV-infected subjects, representing four different stages of HIV disease, and from six HIV-negative controls. Three-colour lymphocyte immunophenotyping was performed using peridinyl chlorophyll-A protein (PerCP)-conjugated anti-CD8 monoclonal antibody (MAb) in combination with anti-HLA-DR (phycoerythrin) and anti-CD38 (fluorescein isothiocyanate) MAb. RESULTS: The relative percentage of the lymphocyte populations thus defined differed between HIV-negative and HIV-positive subjects and between HIV-infected subjects at different clinical stages of disease. Simultaneous expression of HLA-DR and CD38 within the CD8 T-lymphocyte compartment increased from 8% in controls to 49% in asymptomatic HIV-infected subjects (P less than 0.005). Symptomatic patients differed from asymptomatic seropositives by a further increase in the HLA-DR+ CD38+ CD8 subset. In AIDS patients, the HLA-DR+ CD38- CD8 subset decreased (P less than 0.05) and the HLA-DR- CD38+ CD8 subset increased (P less than 0.05), compared with the other HIV disease stage patients. CONCLUSION: There is a stage-associated pattern of HLA-DR and CD38 expression on CD8 T-lymphocytes during HIV infection; specific phenotypic patterns may have functional correlates in the host response to the virus. Link for e-copy: http://journals.lww.com/aidsonline/Abstract/1992/08000/Expression_of_activation_ [...] Format of e-copy: IP recognition ((via CEBAM - personal login recquired) Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2202 [article]

Two-color Immuno-fluorescence and Flow Cytometric Analysis of Peripheral Blood Lymphocyte Subsets in Caucasian and Japanese Healthy Subjects / M. Senju in Japanese journal of medicine, 30(1991)06 ([10/01/1991])  

[article] Two-color Immuno-fluorescence and Flow Cytometric Analysis of Peripheral Blood Lymphocyte Subsets in Caucasian and Japanese Healthy Subjects [printed text] / M. Senju, Author ; K. Makiyama, Author ; K. Hara, Author ; Frank Hulstaert, Author ; N. Lowder, Author ; D.P. Jewell, Author . - 1991 . - 509-15. Languages : English (eng) in Japanese journal of medicine > 30(1991)06 [10/01/1991] . - 509-15 Descriptors: Classification W 1 Serials. Periodicals Indexation Adult ; Antigens ; Asian Continental Ancestry Group ; European Continental Ancestry Group ; Female ; Flow Cytometry ; Fluorescent Antibody Technique ; Humans ; Japan ; Journal Article ; Lymphocyte Subsets ; Male ; Middle Aged Abstract: Two-color immunofluorescence using multiparameter flow cytometry was employed to examine the antigenic characteristics of peripheral blood lymphocytes in whole blood of healthy Caucasians and Japanese. The CD4/CD8 ratio in Japanese was significantly decreased compared with that in Caucasians, because of the increased number of CD8+ cells. Although the proportions of suppressor-inducer T cells (CD4+, Leu-8+) and helper-inducer T cells (naive T cells) (CD4+, CD45RA-) were low in Japanese subjects, there were no differences in the absolute numbers of suppressor-inducer T cells and helper-inducer T cells (naive T cells) in circulation. The level of activated T cells in Japanese was similar to that in Caucasians. NK cells, CD57+, CD8+ cells and CD57+, CD3+ cells were high in Japanese. Regarding B cell subsets, CD5+ B cells and activated B cells remained unchanged. However, there were slight differences in Leu-8+ B cells and Fc epsilon R+ B cells (CD20+, CD23+) between the two groups. Thus, a differing influence of racial and environmental background between healthy Caucasians and healthy Japanese on human lymphocyte subsets is present in the lymphocyte immunophenotype. Link for e-copy: http://www.journalarchive.jst.go.jp/jnlpdf.php?cdjournal=internalmedicine1962&cd [...] Format of e-copy: PDF [Open Access] Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2185 [article]