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Age-related changes in human blood lymphocyte subpopulations / Frank Hulstaert in Clinical immunology and immunopathology, 70(1994)02 ([02/01/1994])  

[article] Age-related changes in human blood lymphocyte subpopulations : II. Varying Kinetics of Percentage and Absolute Count Measurements [printed text] / Frank Hulstaert, Author ; I. Hannet, Author ; V. Deneys, Author ; V. Munhyeshuli, Author ; T. Reichert, Author ; M. De Bruyere, Author ; K. Strauss, Author . - 1994 . - 152-158. Languages : English (eng) in Clinical immunology and immunopathology > 70(1994)02 [02/01/1994] . - 152-158 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Aging ; Antigens, CD ; Antigens, Differentiation ; Child ; Child, Preschool ; Female ; Humans ; Immunophenotyping ; Infant ; Infant, Newborn ; Journal Article ; Leukocyte Count ; Male ; Middle Aged ; T-Lymphocytes ; United States Abstract: A reference range for lymphocyte populations, with particular emphasis on T lymphocyte subsets, was obtained for normal individuals covering age cohorts from birth through adulthood. This report confirms and extends findings from a developmental reference range published earlier (1). Absolute numbers of WBC, lymphocytes, and T, B, and NK subsets decline significantly during childhood. However, differences in the rate of decline of certain lymphocyte subsets leads to discordance between absolute numbers and percentages. Those lymphocyte subsets which decline less rapidly with age than the total lymphocyte count will show an increase in percentage, whereas those which decline more rapidly will show further declines in percentage values. T cell percentages were seen to increase over time whereas B cell percentages decline. Markers of immaturity such as CD45RA on CD4 cells and CD38 on CD8 cells declined in both percentages and absolute numbers. Activation markers, such as HLA-DR on CD8 cells and IL2-R on CD3 cells, increased in percentages with time but changed inconsistently in cell number from infancy to adulthood. These findings extend the lymphocyte references range to markers thought to be informative in various disease states, including HIV infection. Link for e-copy: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCK-45R79S2-9&_user=1 [...] Format of e-copy: VDIC IP recognition Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2209 [article]

A candidate vaccine based on the hepatitis C E1 protein / Leroux-Roels, G in Vaccine, 22(2004)23-24 ([08/13/2004])  

[article] A candidate vaccine based on the hepatitis C E1 protein : tolerability and immunogenicity in healthy volunteers [printed text] / Leroux-Roels, G, Author ; E. Depla, Author ; Frank Hulstaert, Author ; L. Tobback, Author ; S. Dincq, Author ; J. Desmet, Author ; I. Desombere, Author ; G. Maertens, Author . - 2004 . - 3080-6. Languages : English (eng) in Vaccine > 22(2004)23-24 [08/13/2004] . - 3080-6 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Antibody Formation ; Hepatitis C ; Humans ; Immunity, Cellular ; Immunization, Secondary ; Injections, Intramuscular ; Interferon-alpha ; Interleukin-5 ; Journal Article ; Lymphocyte Count ; Male ; Middle Aged ; Netherlands ; Peer Review ; T-Lymphocytes ; Vaccines, Synthetic ; Viral Envelope Proteins ; Viral Vaccines Abstract: The tolerability and immunogenicity of the hepatitis C virus E1 protein as a candidate vaccine was examined in a Phase I, single-arm study. Twenty healthy male volunteers were injected in the deltoid muscle at weeks 0, 3 and 6 with 20 microg recombinant E1 adsorbed on alum. A fourth (booster) dose was administered to 19 subjects at week 26. The candidate therapeutic vaccine was well tolerated. Three vaccine doses induced a clear humoral anti-E1 response that was boosted by a fourth dose. A strong, specific cellular immune response towards E1 was elicited in all vaccine recipients, which included a clear Th1 type response in all but one of the subjects. Link for e-copy: http://tiny.cc/l9e3r Format of e-copy: VDIC IP recognition Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2189 [article]

Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection / L. Kestens in AIDS, 6(1992)8 ([08/01/1992])  

[article] Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection [printed text] / L. Kestens, Author ; G. Vanham, Author ; P. Gigase, Author ; G. Young, Author ; I. Hannet, Author ; F. Vanlangendonck, Author ; Frank Hulstaert, Author ; B.A. Bach, Author . - 1992 . - 793-7. Languages : English (eng) in AIDS > 6(1992)8 [08/01/1992] . - 793-7 Descriptors: Classification W 1 Serials. Periodicals Indexation Antigens, CD ; Antigens, Differentiation ; Fluorescent Antibody Technique ; HIV Infections ; Humans ; Journal Article ; Membrane Glycoproteins ; Peer Review ; T-Lymphocytes ; United States Abstract: OBJECTIVE: To study the expression of the activation markers human leukocyte antigen (HLA)-DR and CD38 antigen on CD8+ T-lymphocytes in HIV-infected subjects and HIV-negative controls. DESIGN: Two- and three-colour flow-cytometric analysis. METHODS: Fresh peripheral venous blood was obtained from 16 HIV-infected subjects, representing four different stages of HIV disease, and from six HIV-negative controls. Three-colour lymphocyte immunophenotyping was performed using peridinyl chlorophyll-A protein (PerCP)-conjugated anti-CD8 monoclonal antibody (MAb) in combination with anti-HLA-DR (phycoerythrin) and anti-CD38 (fluorescein isothiocyanate) MAb. RESULTS: The relative percentage of the lymphocyte populations thus defined differed between HIV-negative and HIV-positive subjects and between HIV-infected subjects at different clinical stages of disease. Simultaneous expression of HLA-DR and CD38 within the CD8 T-lymphocyte compartment increased from 8% in controls to 49% in asymptomatic HIV-infected subjects (P less than 0.005). Symptomatic patients differed from asymptomatic seropositives by a further increase in the HLA-DR+ CD38+ CD8 subset. In AIDS patients, the HLA-DR+ CD38- CD8 subset decreased (P less than 0.05) and the HLA-DR- CD38+ CD8 subset increased (P less than 0.05), compared with the other HIV disease stage patients. CONCLUSION: There is a stage-associated pattern of HLA-DR and CD38 expression on CD8 T-lymphocytes during HIV infection; specific phenotypic patterns may have functional correlates in the host response to the virus. Link for e-copy: http://journals.lww.com/aidsonline/Abstract/1992/08000/Expression_of_activation_ [...] Format of e-copy: IP recognition ((via CEBAM - personal login recquired) Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2202 [article]

Flow cytometric analysis of peripheral blood lymphocytes in ulcerative colitis and Crohn's disease / M. Senju in Gut, 32(1991)7 ([07/01/1991])  

[article] Flow cytometric analysis of peripheral blood lymphocytes in ulcerative colitis and Crohn's disease [printed text] / M. Senju, Author ; Frank Hulstaert, Author ; J. Lowder, Author ; D.P. Jewell, Author . - 1991 . - 779-83. Languages : English (eng) in Gut > 32(1991)7 [07/01/1991] . - 779-83 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Aged ; Antibodies, Monoclonal ; Antigens ; Colitis, Ulcerative ; Crohn Disease ; Female ; Flow Cytometry ; Great Britain ; Humans ; immunology ; Journal Article ; Male ; Middle Aged ; Peer Review ; T-Lymphocytes Abstract: Using two colour immunofluorescence with fluorescein isothiocyanate and phycoerythrin labelled monoclonal antibodies, multi-parameter flow cytometry was used to examine the antigenic characteristics of peripheral blood lymphocytes in whole blood of patients with ulcerative colitis and Crohn's disease who were not taking immunosuppressive drugs. The numbers of CD4+ and CD8+ lymphocytes in patients with ulcerative colitis and Crohn's disease remained unchanged so that the CD4/CD8 ratio was the same as that of normal control subjects. In Crohn's disease there were many activated T cells (CD3+, CD25+). Although natural killer cells in active Crohn's disease were lower than in normal control subjects, cytotoxic T lymphocytes, as defined by CD3+, CD16+, did not differ in patients with inflammatory bowel disease compared with normal control subjects. For B cell subsets, there were differences in Leu-1+ B cells, Leu-8+ B cells, Fc epsilon R+B cells (Leu-16+, Leu-20+), and activated B cells (Leu-12+, Leu-21+) between patients with inflammatory bowel disease and normal control subjects. These differences are compatible with local activation of B cells in the inflamed colon. Link for e-copy: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1378995/pdf/gut00588-0075.pdf Format of e-copy: PDF [Open Access] (850 Kb) Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2183 [article]

Immunologic response of unvaccinated workers exposed to anthrax, Belgium. / Pierre Wattiau in Emerging Infectious Diseases, 15(2009)10 ([10/01/2009])  

[article] Immunologic response of unvaccinated workers exposed to anthrax, Belgium. [printed text] / Pierre Wattiau, Author ; Marc Govaerts, Author ; Dimitrios Frangoulidis, Author ; David Fretin, Author ; Esther Kissling, Author ; Mieke Van Hessche, Author ; Bernard China, Author ; Martin Poncin, Author ; Yvo Pirenne, Author ; Germaine Hanquet, Author . - 2009 . - 1637-40. Languages : English (eng) in Emerging Infectious Diseases > 15(2009)10 [10/01/2009] . - 1637-40 Descriptors: Classification W 1 Serials. Periodicals Indexation Anthrax ; Anthrax Vaccines ; Antibodies, Bacterial ; Bacillus anthracis ; Belgium ; Blotting, Western ; Cell Proliferation ; Enzyme-Linked Immunosorbent Assay ; Humans ; Inhalation Exposure ; Journal Article ; Occupational Exposure ; Peer Review ; T-Lymphocytes ; United States Abstract: To determine immunologic reactivity to Bacillus anthrax antigens, we conducted serologic testing of workers in a factory that performed scouring of wool and goat hair. Of 66 workers, approximately 10% had circulating antibodies or T lymphocytes that reacted with anthrax protective antigen. Individual immunity varied from undetectable to high. Link for e-copy: http://www.cdc.gov/eid/content/15/10/pdfs/1637.pdf Format of e-copy: PDF [Open Access] Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2373 [article]

Iron supplementation in previously anemic Bolivian children normalized hematologic parameters, but not immunologic parameters. / E. Sejas in Journal of Tropical Pediatrics, 54(2008)3 ([06/02/2008])  

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