Author details
Author P. Lancaster |
Available item(s) by this author (1)
Add the result to your basket Make a suggestion Refine your search Apply to external sources
An optimized method for routine HLA-B27 screening using flow cytometry / Frank Hulstaert in Cytometry, 18(1994)1 ([03/15/1994])
[article] An optimized method for routine HLA-B27 screening using flow cytometry [printed text] / Frank Hulstaert, Author ; Johan Albrecht, Author ; I. Hannet, Author ; P. Lancaster, Author ; L. Buchner, Author ; J. Kunz, Author ; A. Falkenrodt, Author ; M. Tongio, Author ; F. De Keyser, Author ; E.M. Veys, Author . - 1994 . - 21-29.
Languages : English (eng)
in Cytometry > 18(1994)1 [03/15/1994] . - 21-29
Descriptors: Classification
W 1 Serials. Periodicals
Indexation
Anticoagulants ; Blood Donors ; Blood Preservation ; Cytotoxicity Tests, Immunologic ; Evaluation Studies as Topic ; Flow Cytometry ; Great Britain ; Humans ; Immune Sera ; Journal Article ; Mass Screening ; Peer Review ; Reproducibility of Results ; Sensitivity and specificityAbstract: Flow cytometry and monoclonal antibodies are promising tools for HLA-antigen detection. Previous approaches have been hampered by the lack of a carefully standardized system for calibration and sample analysis. A new system for HLA-B27 screening was developed using a FACScan flow cytometer, software for automated calibration and analysis, calibration beads, and the anti-HLA-B27-FITC/anti-Leu4-PE (CD3) monoclonal antibodies. The median fluorescence channel result for the HLA-B27-FITC signal of CD3+ T lymphocytes is compared to a decision marker. Values lower than this threshold are read as HLA-B27 negative and those above are recommended for retesting with the classic microcytotoxicity assay on the presumption of HLA-B27 positivity. The anti-HLA-B27 antibody reacts with all six HLA-B27 subtypes and shows a weaker binding to HLA-B7. The screening test results were compared with those from the microcytotoxicity assay for HLA-typing in studies involving several European centers. The observed sensitivity was 100% (95% Cl:98.6-100) and the specificity was 97.4% (95% Cl: 96.4-98.3). Other performance studies verified the reproducibility and reliability of results obtained with the screening system. Link for e-copy: http://onlinelibrary.wiley.com/doi/10.1002/cyto.990180106/pdf Format of e-copy: PDF [Open Access] (Embargo 1 years) Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2211 [article]