Author B.A. Bach
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Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection / L. Kestens in AIDS, 6(1992)8 ([08/01/1992])
[article] Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection [printed text] / L. Kestens, Author ; G. Vanham, Author ; P. Gigase, Author ; G. Young, Author ; I. Hannet, Author ; F. Vanlangendonck, Author ; Frank Hulstaert, Author ; B.A. Bach, Author . - 1992 . - 793-7.
Languages : English (eng)
in AIDS > 6(1992)8 [08/01/1992] . - 793-7
W 1 Serials. Periodicals
Antigens, CD ; Antigens, Differentiation ; Fluorescent Antibody Technique ; HIV Infections ; Humans ; Journal Article ; Membrane Glycoproteins ; Peer Review ; T-Lymphocytes ; United States
Abstract: OBJECTIVE: To study the expression of the activation markers human leukocyte antigen (HLA)-DR and CD38 antigen on CD8+ T-lymphocytes in HIV-infected subjects and HIV-negative controls.
DESIGN: Two- and three-colour flow-cytometric analysis.
METHODS: Fresh peripheral venous blood was obtained from 16 HIV-infected subjects, representing four different stages of HIV disease, and from six HIV-negative controls. Three-colour lymphocyte immunophenotyping was performed using peridinyl chlorophyll-A protein (PerCP)-conjugated anti-CD8 monoclonal antibody (MAb) in combination with anti-HLA-DR (phycoerythrin) and anti-CD38 (fluorescein isothiocyanate) MAb.
RESULTS: The relative percentage of the lymphocyte populations thus defined differed between HIV-negative and HIV-positive subjects and between HIV-infected subjects at different clinical stages of disease. Simultaneous expression of HLA-DR and CD38 within the CD8 T-lymphocyte compartment increased from 8% in controls to 49% in asymptomatic HIV-infected subjects (P less than 0.005). Symptomatic patients differed from asymptomatic seropositives by a further increase in the HLA-DR+ CD38+ CD8 subset. In AIDS patients, the HLA-DR+ CD38- CD8 subset decreased (P less than 0.05) and the HLA-DR- CD38+ CD8 subset increased (P less than 0.05), compared with the other HIV disease stage patients.
CONCLUSION: There is a stage-associated pattern of HLA-DR and CD38 expression on CD8 T-lymphocytes during HIV infection; specific phenotypic patterns may have functional correlates in the host response to the virus.
Link for e-copy: http://journals.lww.com/aidsonline/Abstract/1992/08000/Expression_of_activation_ [...] Format of e-copy: IP recognition ((via CEBAM - personal login recquired) Record link: [article]The significance of activation markers on CD8 lymphocytes in human immunodeficiency syndrome: staging and prognostic value / M. Levacher in Clinical and experimental immunology, 90(1992)3 ([12/01/1992])
[article] The significance of activation markers on CD8 lymphocytes in human immunodeficiency syndrome: staging and prognostic value [printed text] / M. Levacher, Author ; Frank Hulstaert, Author ; S. Tallet, Author ; S. Ullery, Author ; J.J. Pocidalo, Author ; B.A. Bach, Author . - 1992 . - 376-382.
Languages : English (eng)
in Clinical and experimental immunology > 90(1992)3 [12/01/1992] . - 376-382
W 1 Serials. Periodicals
Acquired Immunodeficiency Syndrome ; Adult ; Aged ; AIDS-Related Complex ; Antigens, CD ; Biological Markers ; Female ; Great Britain ; HIV Infections ; HIV Seropositivity ; Humans ; Immunologic Deficiency Syndromes ; Immunophenotyping ; Journal Article ; Lymphocytes ; Male ; Middle Aged ; Peer Review ; Prognosis
Abstract: The objective of this prospective cohort study was to evaluate the expression of activation markers on CD8 lymphocytes at various clinical stages of HIV infection and to determine the value of these markers in identifying patients likely to have rapidly progressive disease. One hundred and three HIV+ patients, divided into four disease stages, and 34 seronegative controls were evaluated at study entry using flow cytometric immunophenotyping. The HIV patients were followed clinically for disease progression during the following 2 years. CD8 cell numbers and percentage of lymphocytes are increased after HIV infection. Expression of the CD38, HLA-DR and CD57 markers on CD8 cells was significantly increased in asymptomatic HIV-infected patients when compared with controls, as was the CD8 cell population which did not coexpress Leu-8. These activation markers were observed to be further increased in patient groups with more clinically advanced infection. The percentage of CD38 on CD8 cells emerged not only as a discriminator of disease severity, but was a strong predictor of progression in asymptomatic, lymphadenopathy and ARC patients. Given the utility of activation markers on CD8 lymphocytes in staging disease and predicting clinical outcome, the measurement of these parameters should be considered in the monitoring and management of HIV patients. Link for e-copy: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1554578/pdf/clinexpimmunol00044-0024 [...] Format of e-copy: VDIC IP recognition (1966 to embargo 1 year) Record link: [article]