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Age-related changes in human blood lymphocyte subpopulations / F. Erkeller-Yuksel in The Journal of Pediatrics, 120(1996)2 ([02/01/1996])  

[article] Age-related changes in human blood lymphocyte subpopulations [printed text] / F. Erkeller-Yuksel, Author ; V. Deneys, Author ; B. Yuksel, Author ; I. Hannet, Author ; Frank Hulstaert, Author ; C. Hamilton, Author . - 1996 . - 216-222. Languages : English (eng) in The Journal of Pediatrics > 120(1996)2 [02/01/1996] . - 216-222 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Aged ; Aging ; Antigens, CD ; Child ; Child, Preschool ; Female ; Fetal Blood ; Humans ; Immunophenotyping ; Infant ; Journal Article ; Leukocyte Count ; Lymphocyte Subsets ; Male ; Middle Aged ; Peer Review ; United States Abstract: Flow cytometric analysis of major lymphocyte populations and their subsets reveals age-related changes in the cellular human immune system. Immunophenotypic markers were evaluated in 110 normal pediatric subjects, divided into groups of newborn infants, infants aged 2 days to 11 months, and children aged 1 to 6 years and 7 to 17 years; results were then compared with those obtained from 101 normal adults aged 18 to 70 years. Comparisons among age groups from newborn infants through adults reveal progressive declines in the absolute numbers of leukocytes, total lymphocytes, and T, B, and natural killer (NK) cells. The percentages of T cells within the total lymphocyte population increase with age, in both CD4+ and CD8+ subsets. Percentages of B and NK cells are higher in newborn infants than in adults. The expression of the activation markers interleukin-2R and HLA-DR on T cells increases with age, as does the NK-associated expression of CD57 on CD8 cells. The proportions of B lymphocytes that coexpress CD5 or CDw78 decrease with age, whereas expression of Leu-8 and CD23 increases. The proportion of CD4 cells bearing the CD45RA and Leu-8 markers is consistently lower in adults than in children. These data may serve as a reference range for studies of pediatric subjects. Link for e-copy: http://www.sciencedirect.com/science/journal/00223476 Format of e-copy: PDF [Requires Subscription] Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2187 [article]

Age-related changes in human blood lymphocyte subpopulations / Frank Hulstaert in Clinical immunology and immunopathology, 70(1994)02 ([02/01/1994])  

[article] Age-related changes in human blood lymphocyte subpopulations : II. Varying Kinetics of Percentage and Absolute Count Measurements [printed text] / Frank Hulstaert, Author ; I. Hannet, Author ; V. Deneys, Author ; V. Munhyeshuli, Author ; T. Reichert, Author ; M. De Bruyere, Author ; K. Strauss, Author . - 1994 . - 152-158. Languages : English (eng) in Clinical immunology and immunopathology > 70(1994)02 [02/01/1994] . - 152-158 Descriptors: Classification W 1 Serials. Periodicals Indexation Adolescent ; Adult ; Aging ; Antigens, CD ; Antigens, Differentiation ; Child ; Child, Preschool ; Female ; Humans ; Immunophenotyping ; Infant ; Infant, Newborn ; Journal Article ; Leukocyte Count ; Male ; Middle Aged ; T-Lymphocytes ; United States Abstract: A reference range for lymphocyte populations, with particular emphasis on T lymphocyte subsets, was obtained for normal individuals covering age cohorts from birth through adulthood. This report confirms and extends findings from a developmental reference range published earlier (1). Absolute numbers of WBC, lymphocytes, and T, B, and NK subsets decline significantly during childhood. However, differences in the rate of decline of certain lymphocyte subsets leads to discordance between absolute numbers and percentages. Those lymphocyte subsets which decline less rapidly with age than the total lymphocyte count will show an increase in percentage, whereas those which decline more rapidly will show further declines in percentage values. T cell percentages were seen to increase over time whereas B cell percentages decline. Markers of immaturity such as CD45RA on CD4 cells and CD38 on CD8 cells declined in both percentages and absolute numbers. Activation markers, such as HLA-DR on CD8 cells and IL2-R on CD3 cells, increased in percentages with time but changed inconsistently in cell number from infancy to adulthood. These findings extend the lymphocyte references range to markers thought to be informative in various disease states, including HIV infection. Link for e-copy: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCK-45R79S2-9&_user=1 [...] Format of e-copy: VDIC IP recognition Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2209 [article]

An optimized method for routine HLA-B27 screening using flow cytometry / Frank Hulstaert in Cytometry, 18(1994)1 ([03/15/1994])  

[article] An optimized method for routine HLA-B27 screening using flow cytometry [printed text] / Frank Hulstaert, Author ; Johan Albrecht, Author ; I. Hannet, Author ; P. Lancaster, Author ; L. Buchner, Author ; J. Kunz, Author ; A. Falkenrodt, Author ; M. Tongio, Author ; F. De Keyser, Author ; E.M. Veys, Author . - 1994 . - 21-29. Languages : English (eng) in Cytometry > 18(1994)1 [03/15/1994] . - 21-29 Descriptors: Classification W 1 Serials. Periodicals Indexation Anticoagulants ; Blood Donors ; Blood Preservation ; Cytotoxicity Tests, Immunologic ; Evaluation Studies as Topic ; Flow Cytometry ; Great Britain ; Humans ; Immune Sera ; Journal Article ; Mass Screening ; Peer Review ; Reproducibility of Results ; Sensitivity and specificity Abstract: Flow cytometry and monoclonal antibodies are promising tools for HLA-antigen detection. Previous approaches have been hampered by the lack of a carefully standardized system for calibration and sample analysis. A new system for HLA-B27 screening was developed using a FACScan flow cytometer, software for automated calibration and analysis, calibration beads, and the anti-HLA-B27-FITC/anti-Leu4-PE (CD3) monoclonal antibodies. The median fluorescence channel result for the HLA-B27-FITC signal of CD3+ T lymphocytes is compared to a decision marker. Values lower than this threshold are read as HLA-B27 negative and those above are recommended for retesting with the classic microcytotoxicity assay on the presumption of HLA-B27 positivity. The anti-HLA-B27 antibody reacts with all six HLA-B27 subtypes and shows a weaker binding to HLA-B7. The screening test results were compared with those from the microcytotoxicity assay for HLA-typing in studies involving several European centers. The observed sensitivity was 100% (95% Cl:98.6-100) and the specificity was 97.4% (95% Cl: 96.4-98.3). Other performance studies verified the reproducibility and reliability of results obtained with the screening system. Link for e-copy: http://onlinelibrary.wiley.com/doi/10.1002/cyto.990180106/pdf Format of e-copy: PDF [Open Access] (Embargo 1 years) Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2211 [article]

Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection / L. Kestens in AIDS, 6(1992)8 ([08/01/1992])  

[article] Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection [printed text] / L. Kestens, Author ; G. Vanham, Author ; P. Gigase, Author ; G. Young, Author ; I. Hannet, Author ; F. Vanlangendonck, Author ; Frank Hulstaert, Author ; B.A. Bach, Author . - 1992 . - 793-7. Languages : English (eng) in AIDS > 6(1992)8 [08/01/1992] . - 793-7 Descriptors: Classification W 1 Serials. Periodicals Indexation Antigens, CD ; Antigens, Differentiation ; Fluorescent Antibody Technique ; HIV Infections ; Humans ; Journal Article ; Membrane Glycoproteins ; Peer Review ; T-Lymphocytes ; United States Abstract: OBJECTIVE: To study the expression of the activation markers human leukocyte antigen (HLA)-DR and CD38 antigen on CD8+ T-lymphocytes in HIV-infected subjects and HIV-negative controls. DESIGN: Two- and three-colour flow-cytometric analysis. METHODS: Fresh peripheral venous blood was obtained from 16 HIV-infected subjects, representing four different stages of HIV disease, and from six HIV-negative controls. Three-colour lymphocyte immunophenotyping was performed using peridinyl chlorophyll-A protein (PerCP)-conjugated anti-CD8 monoclonal antibody (MAb) in combination with anti-HLA-DR (phycoerythrin) and anti-CD38 (fluorescein isothiocyanate) MAb. RESULTS: The relative percentage of the lymphocyte populations thus defined differed between HIV-negative and HIV-positive subjects and between HIV-infected subjects at different clinical stages of disease. Simultaneous expression of HLA-DR and CD38 within the CD8 T-lymphocyte compartment increased from 8% in controls to 49% in asymptomatic HIV-infected subjects (P less than 0.005). Symptomatic patients differed from asymptomatic seropositives by a further increase in the HLA-DR+ CD38+ CD8 subset. In AIDS patients, the HLA-DR+ CD38- CD8 subset decreased (P less than 0.05) and the HLA-DR- CD38+ CD8 subset increased (P less than 0.05), compared with the other HIV disease stage patients. CONCLUSION: There is a stage-associated pattern of HLA-DR and CD38 expression on CD8 T-lymphocytes during HIV infection; specific phenotypic patterns may have functional correlates in the host response to the virus. Link for e-copy: http://journals.lww.com/aidsonline/Abstract/1992/08000/Expression_of_activation_ [...] Format of e-copy: IP recognition ((via CEBAM - personal login recquired) Record link: https://kce.docressources.info/index.php?lvl=notice_display&id=2202 [article]